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Department of Biomedical Sciences

 

 

 
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邱健泰(Jiantai Timothy Qiu)

Jian-Tai Timothy Qiu

Position

Assistant Professor

Education

Ph.D., Department of Molecular Microbiology and Immunology, the Johns Hopkins University, USA

E-mail

jtqiu@mail.cgu.edu.tw

Office Tel

+886-3-211-8800 ext.3494

Fax

+886-3-211-8700

Laboratory

 

Spciality

Immunology and Microbiology

Lab & Research Interest

1. Study  the  immune  response of  T  lymphocyte  to  a high-dose GM-CSF-secreting  tumor  cell-based vaccine in mice

We had modified  codon   usage   of granulocyte-macrophages stimulating factor (GM-CSF) of mice but without changing amino acid sequence. As a consequent of modifying codon, it improved the stability of the mRNA, thereby  enhancing  the  expression  amount  of  the  protein.  By  using  lentivirus  vectors,  we transfected  mcGM-CSF  into  HPV  oncogenic  E6/E7  of  mouse  tumor  cells,  TC-1.  Since GM-CSF  is  an  important  hematopoietic  growth  factor,  activating  the  immune  system, including T cells and dendritic cells,  will be used as a vaccine adjuvant.  In this  project, we will   vaccinate    radiated   TC-1,   TC-1/wtGM   (wild-type   GM-CSF)   or   TC-1/cGM (codon-optmized GMCSF) in mice, and analysis antigen-specific CD8 + T cells and dendritic cells response by flow cytometry. In addition, some papers indicated that several vaccinations will induce suppressor T cells and MDSC cells. In this project, we will also analyze whether the same phenomenon will be occurred at and to investigate the mechanism of this induction.   We had studied the DC and CD4 cells activity in IL-15Ra-/- mice and published two papers on  this  area.  We  will  apply  the  IL-15Ra-/-  mice  in  cell  base  vaccine  model  to  dissect  the immune regulations in cancer tolerance.

2. Rapid and sensitive human papillomavirus genotyping by high resolution melting analysis (1) to establish a fast and cost-effective high resolution melting assay system for the detection of the clinically most relevant high-risk HPV types and (2) to tested whether high resolution melting assay system will be able to be useful tool to address questions related to viral persistence at the genotype level, the kinetics of viral load and disease recurrence.

3. Human clinical trials --- Targeted therapy and Immunotherapy

Publications

Publications:

  1. Huang CC, Qiu JT, Kashima ML, Kurman RJ, Wu TC* Generation of Type-Specific Probes for the Detection of Single Copy Human Papilloma Virus by a Novel In Situ Hybridization Method, Modern Pathology 1998 Oct. 11(10): 971-7. (SCI; IF:4.29; Pathology 8/78)
  2. Qiu JT, Liu B, Tian C, Pavlakis GN, Yu XF*  Enhancement primary and secondary cellular immune responses against HIV-1 Gag by DNA expression vectors that target Gag antigen to the secretory pathway. J Virology; 2000 July 74(13): 5997-6005. (SCI; IF:5.40; Virology 6/32)
  3. Qiu JT, Song R, Dettenhofer M, Tian C, August T, Felber BK, Pavlakis GN, Yu XF*  Evaluation of novel human immunodeficiency virus type 1 Gag DNA vaccines for protein expression in mammalian cells and induction of immune responses J Virology;1999 Nov. 73(11): 9145-52. (SCI; IF:5.40; Virology 6/32)
  4. The Johns Hopkins博士學位論文(2001年度) DNA Vaccine against HIV-1 Gag-- From the bench to the field 抗愛滋病毒之核酸疫苗:從實驗桌到田野試驗
  5. Cheng PJ*, Chueh HY, Qiu JT. Heterotopic pregnancy in a natural conception cycle presenting as hematometra. Obstet Gynecol. 2004 Nov; 104(5 Pt 2):1195-8. (SCI; IF:4.73; Obs & Gyn 2/79)
  6. Huang SL, Chao A, Hsueh S, Chao FY, Huang CC, Yang JE, Lin CY, Yan CC, Chou HH, Huang KG, Huang HJ, Wu TI, Tseng MJ, Qiu JT, Lin CT, Chang TC, Lai CH*. Comparison between the Hybrid Capture II Test and an SPF1/GP6+ PCR-based assay for detection of  human papillomavirus DNA in cervical swab samples. J Clin Microbiol. 2006 May; 44 (5):1733-9. (SCI; IF:4.15; Microbiology 24/112)
  7. Qiu JT*, Chang TC, Lin CT, Chen YM, Li FQ, Soong YK, Lai CH*. Novel codon-optimized GM-CSF gene as an adjuvant to enhance the immunity of a DNA vaccine against HIV-1 Gag. Vaccine. 2007 Jan 4;25(2):253-63 (SCI; IF:3.77; Medicine, Research & Experimental 23/111)
  8. Lai CH*, Huang HJ, Hsueh S, Chao A, Lin CT, Huang SL, Chao FY, Qiu JT, Hong JH, Chou HH, Chang TC, Chang CJ. Human papillomavirus genotype in cervical cancer: a population-based study. Int J Cancer. 2007 May 1; 120(9):1999-2006. (SCI; IF:5.44; Oncology  26/194)
  9. Qiu JT, Ho KC, Lai CH*, Yen TC, Huang YT, Chao A, Chang TC. Supraclavicular lymph node metastases in cervical cancer. Eur J Gynaecol Oncol. 2007; 28(1): 33-8. (SCI; IF:0.59; Oncology  69/79)
  10. Chao A, Jao MS, Huang CC, Huang HJ, Cheng HH, Yang JE, Hsueh S, Chen TC, Qiu JT, Lin CT, Fu CJ, Chou HH, Lai CH*. Human papillomavirus genotype in cervical intraepithelial neoplasia grades 2 and 3 of Taiwanese women. Int J Cancer. 2011 Feb 1; 128(3):653-9. (SCI; IF:5.44; Oncology 26/194)
  11. Ho KC, Wang CC, Qiu JT, Lai CH, Hong JH, Huang YT, Huang KG, Chao A, Lin G, Yen TC*. Identification of prognostic factors in patients with cervical cancer and supraclavicular lymph node recurrence. Gynecol Oncol. 2011 Jul 30. 123(2):253-256 (SCI; IF:3.88; Obs/Gyn 13/59)
  12. Qiu JT, Chow KP, Lee JM, Liao SK, Lin SC, Shu SL, Wu NN, Lin YF, Wu TS*. Enhanced IL-10 production by CD4 (+) T cells primed in IL-15Rα deficient mice. Eur J Immunol. 2011 Aug 23. 41(11):3146~3156 (SCI; IF:5.10; Immunology 24/139)
  13. Chiang YC, Qiu JT, Chang CL, Wang PH, Ho CM, Lin WC, Huang YF, Lin H, Lu CH, Chou CY*. Brain metastases from epithelial ovarian carcinoma: evaluation of prognosis and managements - a Taiwanese Gynecologic Oncology Group (TGOG) study. Gynecol Oncol. 2012 Apr; 125(1):37-41. (SCI; IF:3.88; Obs/Gyn 13/59)
  14. Qiu JT, Chow KP, Lee JM, Hsu SL, Yang SC, Wu NN, Huang W, Wu TS*. Selective reduction of post-selection CD8 thymocyte proliferation in IL-15Rα deficient mice. PLoS One. 2012;7(3):e33152 (SCI; IF:4.09; Biology 12/84)
  15. Chen MC, Chen HY, Lin CY, Chien CH, Hsieh TF, Horng JT, Qiu JT, Huang CC, Ho CH, Yang FL*. A CMOS-compatible poly-Si nanowire device with hybrid sensor/memory characteristics for System-on-Chip applications. Sensors (Basel). 2012; 12(4):3952-63. (SCI; IF:1.74; Instruments & Instrumentation 14/58)
  16. Lee TH, Wu TS, Tseng CP, Qiu JT*.High-resolution melting molecular signatures for rapid identification of human papillomavirus genotypes. PLoS One. 2012;7(8):e42051 (SCI; IF:4.09; Biology 12/84) 
  17. Qiu JT, Abdullah NA, Chou HH, Lin CT, Jung SM, Wang CC, Chen MY, Huang KG, Chang TC, Lai CH*. Outcomes and prognosis of patients with recurrent cervical cancer after radical hysterectomy. Gynecol Oncol. 2012 Aug 14 (SCI; IF:3.88; Obs/Gyn 13/59)
  18. Lai CH*, Chou HH, Chang CJ, Wang CC, Hsueh S, Huang YT, Chen YR, Chang HP, Chang SC, Lin CT, Chao A, Qiu JT, Huang KG, Chen TC, Jao MS, Chen MY, Liou JD, Huang CC, Chang TC, Patsner B. Clinical implications of human papillomavirus genotype in cervical adeno-adenosquamous carcinoma. Eur J Cancer. 2012 Sep 29.