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皮海薇(Hai-wei Pi)

皮海薇 (Hai-Wei Pi)

職稱

副教授

最高學歷

美國紐約州立大學
(Ph.D. SUNY at Stony Brook, USA)

E-mail

haiwei@mail.cgu.edu.tw

電話:

+886-3-211-8800 ext.3361

傳真

+886-3-211-8700

實驗室

發育及幹細胞生物學實驗室

專長

發育及幹細胞生物學

研究方向及研究室特色

我們實驗室主要研究細胞分化的過程。在發育過程中,胚胎細胞藉由分化過程來形成各式功能的細胞。分化作用也會在成體中發生,並對成體組織代謝及恆定極為重要。因此,研究細胞分化不只有助於了解及治療因細胞功能不足而產生的先天性疾病,且直接有助於未來在醫學上產生新生細胞來修補受損組織。

我們主要用果蠅這個模式生物來研究在活體中,基因如何調控細胞分化。實驗室中有兩個主要方向:(1)探討神經母細胞的形成過程中,原神經蛋白如何活化下游基因來促進母細胞分化。我們目前著重於探討核內肌動蛋白和原神經蛋白之間的關係。(2)成體幹細胞 (adult stem cell) 自我新生的調控在 維持組織恆定中扮演 重要功能 。自我新生能力太強時, 細胞分化會被抑制。 反之, 過早進入細胞分化會降低幹細胞的自我新生。我們實驗室最近的研究在探討成體中的配子幹細胞如何受到BMP 或其它訊息傳導路徑的影響來維持在自我新生及細胞分化中的協調。

(A)果蠅的翅膀 (B)感覺神經剛毛(箭頭)位於果蠅前翅脈邊緣,負責感覺外界機械性刺激 (C)果蠅成體testis前端,圍繞在hub(中心白色細胞)周圍的細胞為成體配子幹細胞(箭頭)

 

近五年計劃:

行政院國家科學委員會:

2008/8/1~2011/7/31 Phyllopod調節蛋白質降解作用在果蠅神經發育的功能

2011/8/1~2012/7/31 COP9複雜亞單位與核內肌動蛋白對果蠅感覺器官的分布及命運決定之影響

2012/8/1~2015/7/31 探討COP9複雜亞單位對於果蠅蛻皮激素訊息傳遞鏈的抑制機轉

長庚醫院:

2009/12/1~2012/11/30 在果蠅精子幹細胞及子細胞發育中,TGF-β訊息傳遞的副調控和Notch訊息路徑的功能

2013/1/1~2015/12/31 老化果蠅雄性生質幹細胞系統中,smurf扮演抑制細胞過度增生的角色

論文與著作

Publications (* Corresponding author):

1. Kao SH, Tseng CYWan CLSu YHHsieh CCPi HHsu HJ*. (2014) Aging and insulin signaling differentially control normal and tumorous germline stem cells. Aging Cell doi:10.1111/acel.12288.

2. Huang YC, Lu YN, Wu JT, Chien CT*, Pi H*. (2014) The COP9 Signalosome Converts Temporal Hormone Signaling to Spatia Restriction on Neural Competence. PLoS Genetics 10(11):1004760.

3. Hsiao YL, Chen YJ, Chang YJ, Yeh HF, Huang YC and Pi H*. (2014) Proneural proteins Achaete and Scute associate with nuclear actin to promote external sensory organ formation. Journal of Cell Science 127:182-90.

4. Chang YJ†, Pi H†*, Hsieh CC, Fuller MT. (2013) Smurf-mediated differential proteolysis generates dynamic BMP signaling in germline stem cells during Drosophila testis development. Developmental Biology 383(1):106-20. (†These two authors contributed equally to this work).

5. Yang WK, Peng YH, Li H, Lin HC, Lin YC, Lai TT, Suo H, Wang CH, Lin WH, Ou CY, Zhou X, Pi H, Chang HC, Chien CT.* (2011) Nak regulates localization of clathrin sites in higher-order dendrites to promote local dendrite growth. Neuron 72, 285-299.

6. Pi H*, Huang YC, Chen C, Lin CD, Yeh HF, Pai LM. (2011) Identification of 11-amino acid peptides that disrupt Notch-mediated processes in Drosophila. Journal of Biomedical Science 18, 42. (These two authors contributed equally to this work).

7. Wu JT, Lin WH, Chen WY, Huang YC, Tang CY, Ho MS., Pi H, Chien CT*. (2011) CSN-mediated deneddylation differentially modulates Ci155 proteolysis to promote Hedgehog signalling responses. Nature Communications 2,182.

6. Pi H, Lee LW, Lo SJ*. (2009) New Insights into Polycistronic Transcripts in Eukaryotes. Chang Gung Med J. 32, 494-8.

7. Chang PJ, Hsiao YL, Tien AC, Li YJ, Pi H*. (2008) Negative feedback regulation of proneural proteins controls the timing of neural precursor division. Development 135, 3021-30. (Recommended by Dr. Patricia Simpson in  Faculty of 1000).

8. Ho MS, Ou CY, Chan Y, Chien CT.*, Pi H*. (2008) The utility F-box for protein destruction. Cellular and Molecular Life Science 65, 1977-2000.

9. Pi H and Chien CT.* (2007) Getting the edge: neural precursor selection. Journal of Biomedical Science 14, 467-473.

10. Pi H, Huang SK, Tang CY, Sun H, Chien CT*. (2004) phyllopod is a target gene  of proneural protein in Drosophila external sensory organ development. PNAS. 101, 8378-83.

11. Chang CW, Pi H, Chien CT, Hsu CP*. (2003) Network modeling of Drosophila external sensory organ precursor formation: The role of recently studied genes. Journal of Genetics and Molecular Biology. (Taiwan) 14, 243-251

12. Ou CY, Pi H, Chien CT*. (2003) Control of protein degradation by E3 ubiquitin ligases in Drosophila eye development. Trends in Genetics 19, 353-414.

13. Pi H, Wu HJ, Chien CT*. (2001) A dual function of phyllopod in Drosophila external sensory organ development: cell fate specification of sensory organ precursor and its progeny. Development 128, 2699-2710.

14. Pi H, Chien CT, Fields S*. (1997) Transcriptional activation upon pheromone stimulation mediated by a small domain of Saccharomyces cerevisiae Ste12p. Molecular and Cellular Biology 17, 6410-6418.