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陳怡婷(Yi-Ting Chen)

陳怡婷 (Yi-Ting Chen)

職稱

助理教授

最高學歷

清華大學化學所博士

E-mail

ytchen@mail.cgu.edu.tw

電話:

+886-3-211-8800 
ext.3558(office),3563(Lab)

傳真

+886-3-211-8700

實驗室

蛋白質及生化質譜研究室

專長

生化質譜、轉譯蛋白體學、代謝體學、泌尿腫瘤學

研究方向及研究室特色

我們實驗室之研究方向為開發偵測及定量物生物分子(包括蛋白質及代謝物)之質譜技術,這些方法已經被用來研究細胞模式及臨床組織及體液等真實樣品,並經由國際合作保持技術的新穎性。我們的研究目標在於利用本實驗室之專長(蛋白質體、代謝體及生物質譜技術)了解泌尿系統癌症之生物機制,利用體學的方法研究膀胱癌及腎臟癌細胞株、腫瘤組織及尿液,希望找尋早期診斷癌症之非侵入式生物標記。
並與泌尿科、腎臟科、婦產科、小兒科等臨床醫生合作,運用蛋白質體及代謝體等後基因體學研究方法,全面性的研究正常與疾病生物系統(細胞株及臨床收集的體液或組織)中蛋白質與代謝物之群體變化,監測各個分子在疾病所扮演的角色,探討惡性與良性泌尿系統疾病發生及惡化之生物機制,以期朝向臨床應用及個人化醫療邁進。

 

目前實驗室的研究方向如下 :

1. 以系統性方法找尋及驗證泌尿系統癌症之生物標誌及其生物重要性

(Using a Systems Approach to Discover and Assess Urological Cancer Biomarkers)

   我們研究了膀胱癌細胞株之分泌蛋白質體以及臨床醫生取得之腫瘤及尿液組成蛋白體,經由交叉比對,已經發現一系列可能隨著癌症的發生而產生表現量改變的分子,未來將持續驗證其重要性,並研究其與病理狀況之相關性,了解其在泌尿系統研症所扮演之角色。

System approaches for biomarker discovery and mechanism study of urological cancer

2. 發展標靶性及多重定量特性之MRM-MS技術在大量臨床樣品中驗證疾病生物標誌之重要性 (Targeted and Multiplexed Quantitation of Proteins for Verification of Biomarker Panels using Multiple-Reaction-Monitoring MS (MRM-MS) and immune MRM-MS. Collaboration with Prof. Christoph Borchers, the Director of the University of Victoria Genome BC Proteomics Centre, Canada; and clinical teams in Chang Gang Memorial Hospital)

蛋白質體學是當今疾病生物標誌研究中最熱門的研究領域之一我們研究團隊過去的研究成功地iTRAQ技術分析發現膀胱癌病患尿液檢體中多種蛋白質生物標誌,同時也已建立「液相層析多重反應監測質譜定量分析技術」(LC-MRM-MS),首度完成於人類臨床尿液樣本中同步定量63個蛋白質分子的研究工作,此技術將可協助研究學者在大量檢體中驗證過去所找到的生物標記後選分子,未來將繼續發展「免疫濃縮-液相層析多重反應監測質譜定量分析技術」(immuno-MRM-MS)及生物資訊分析方法,以擴展至其他疾病或不同型態的檢體。

Development of MRM-MS assays for protein biomarker verification

3. 利用標靶定量技術同時分析生物路徑中之多種蛋白質及代謝物分子,以系統生物學的方法闡明疾病發生及進展過程中所參與的生物反應機制

(Development and Applications of Targeted Proteomic and Metabolomic Approaches in Cancer Biology to study Urological Oncology. Collaboration with Prof. Liang Li, Department of Chemistry, University of Alberta, Canada)

隨著標靶定量技術的發展,我們開始可以利用質譜方法大規格的追蹤參與特定生物反應機制之多種蛋白質及代謝分子,比較細胞模式在不同處理條件下的表現量,監測各個分子在疾病所扮演的角色,並在個人臨床樣品中驗證,以期朝向個人化醫療邁進。

論文與著作

發表論文:

  1. “MALDI-TOF MS”Yi-Ting ChenY. C. Ling, Instruments Today, 19, 60-70, 1997.
  2. Y. C. Ling, L. Lin and Y. T. Chen, ²Quantitative Analysis of Antibiotics by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry², Rapid Commun. Mass Spectrometry. 12, 317-327, 1998 (SCI).
  3. Y. T. Chen and Y. C. Ling, ²An Overview of Supercritical Fluid Extraction in Chinese Herbal Medicine:from Preparation to Analysis², Journal of Food and Drug Analysis, Special Issue on Chinese Herbal Medicines, 8, 235-247, 2000 (SCI).
  4. Y. T. Chen and Y. C. Ling, ²Detection of Water-soluble Vitamins by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry using Porphyrin Matrices², J. Mass. Sepctrom. 37, 716-730, 2002 (SCI).
  5. Y. T Chen, C. Y. Tsao, J. M Li, C. Y. Tsai, S. F. Chiu, T. L. Tseng*, ² Large-scale Protein Identification of Human Urine Proteome by Multi-dimensional Liquid Chromatography and Tandem Mass Spectrometry ², Proteomics Clin. Appl. 1, 577-587, 2007 (SCI).
  6. Hui-Chu Hsieh, Yi-Ting Chen, Jen-Ming Li, Ting-Yu Chou, Ming-Fong Chang, See-Chang Huang, Tzu-Ling Tseng, Chung-Cheng Liu, and Sung-Fang Chen*, “Protein Profilings in Mouse Liver Regeneration after Partial Hepatectomy using iTRAQ Technology”. J. Proteome Res. 8, 1004-1043, 2009 (SCI).
  7. Yi-Ting Chen*, Chien-Lun Chen, Hsiao-Wei Chen, Ting Chung, Chih-Ching Wu, Chi-De Chen, Chia-Wei Hsu, Meng-Chieh Chen, Ke-Hung Tsui, Phei-Lang Chang, Yu-Sun Chang, Jau-Song Yu*, ² Discovery of Novel Bladder Cancer Biomarkers by Comparative Urine Proteomics Using iTRAQ Technology ², J. Proteome Research. 9, 5803-5815, 2010 (SCI).
  8. Shih-Yuan Chen, Yi-Ting Chen, Jey-Jau Lee, Soofin Cheng*, Tuning Pore Diameter of Platelet SBA-15 Materials for Enzyme Adsorption, J. Mater. Chem. 21, 5693-5703. 2011 (SCI).
  9. Yi-Ting Chen, Hsiao-Wei Chen, Derek Smith, Kung-Hao Liang, Ting Chung, Chien-Lun Chen, Yu-Sun Chang, Christoph Borchers,* Jau-Song Yu.* Multiplexed quantification of 63 proteins in human urine by multiple reaction monitoring-based mass spectrometry for discovery of potential bladder cancer biomarkers. J. Proteomics. 75, 3529-3545, 2012 (SCI).
  10. Yi-Ting Chen*, Fu-Shing Wang, Zhendong Li, Liang Li*, Yong-Chien Ling*. Development of a Matrix-assisted Laser Desorption Ionization Mass Spectrometric Method for Rapid Process-Monitoring of Phthalocyanine Compounds. Analytica Chimica Acta. 736, 69–77, 2012. (SCI)
  11. Chien-Lun Chen, Yue-Fan Lai,, Kun-Yi Chien, Jau-Song Yu, Hsiao-Wei Chen, Petrus Tang, Chia-Wei Hsu, Chi-De Chen, Yu-Sun Chang, Phei-Lang Chang, Yi-Ting Chen*, Comparative and Targeted Proteomic Analyses of Urinary Exosomes from Bladder Cancer and Hernia Patients. J. Proteome Research. 11, 5611−5629, 2012. (SCI)
  12. Yen-Heng Lin*, Ying-Ju Chen, Chao-Sung Lai*, Yi-Ting Chen, Chien-Lun Chen, Jau-Song Yu, and Yu-Sun Chang, A negative-pressure-driven microfluidic chip for the rapid detection of a bladder cancer biomarker in urine using bead-based enzyme-linked immunosorbent assay. Biomicrofluidics 7, 024103, 2013. (SCI)
  13. Chien-Lun Chen, Tsung-Shih, Lin, Cheng-Han, Tsai, Chih-Ching Wu, Ting Chung,  Kun-Yi Chien, Maureen Wu, Yu-Sun Chang, Jau-Song Yu*, Yi-Ting Chen*, Identification of Potential Bladder Cancer Markers in Urine by Abundant-Protein Depletion Coupled with Quantitative Proteomics. J. Proteomics. 85, 28-43, 2013. (SCI)
  14. Chi-De Chen, Chih-Liang Wang, Chia-Jung Yu, Kun-Yi Chien, Yi-Ting Chen, Min-Chi Chen, Yu-Sun Chang, Chin-Ching Wu*, Jau-Song Yu*. Targeted Proteomics Pipeline Reveals Potential Biomarkers forthe Diagnosis of Metastatic Lung Cancer in Pleural Effusion. J. Proteome Research. 13(6):2818-29, 2014.
  15. Jun Peng, Yi-Ting Chen, Chien-Lun Chen and Liang Li, Development of a Universal Metabolome-Standard Method for Long-term LC-MS Metabolome Profiling and Its Application for Bladder Cancer Urine-Metabolite-Biomarker Discovery. Anal. Chem. 86(13):6540-7, 2014.
  16. Chiun-Gung Juo*, Chien-Lun Chen, Shiang-Ting Lin, Shu-Hsuan Fu, Yi-Ting Chen, Yu-Sun Chang and Jau-Song Yu. Mass accuracy improvement of ESI-RPLC-MS based urinary metabolomic analysis by post-run calibration using sodium formate cluster ions. Rapid Commun. Mass Spectrom. 28, 1813-1820, 2014.
  17. Hsiao-Chien Chen, Yi-Ting Chen, Rung-Ywan Tsai, Min-Cheng Chen, Shi-Liang Chen, Min-Cong Xiao, Chien-Lun Chen, Mu-Yi Hua*. A sensitive and selective magnetic graphene composite-modified polycrystalline-silicon nanowire field-effect transistor for bladder cancer diagnosis. Biosensors and Bioelectronics. 66, 198–207, 2015 . (Co-first author)
  18. Yi-Ting Chen, Li-Ping Tuan, Hsiao-Wei Chen, I-An Wei, Min-Yuan Chou, Han-Min Chen,  Yu-Chang Tyan, Sung-Fang Chen*. Quantitative analysis of prostate specific antigen isoforms using immunoprecipitation and isotope dilution mass spectrometry. Anal. Chem. 2015, 87(1):545-53. (Co-first author)
  19. Chien-Lun Chen, Ting Chung, Chih-Ching Wu, Kwai-Fong Ng, Jau-Song Yu, Cheng-Han Tsai, Yu-Sun Chang, Ying Liang, Yi-Ting Chen*. Comparative tissue proteomics of microdissected specimens reveals novel biomarkers of bladder cancer. Mol. Cell Proteomics. 2015. 14(9), 2466-2478. (Co-first and corresponding author)
  20. Chih-Hsiang Chang, Chia-Hung Yang, Huang-Yu Yang, Tien-Hsing Chen, Chan-Yu Lin, Su-Wei Chang, Yi-Ting Chen, Cheng-Chieh Hung, Ji-Tseng Fang, Chih-Wei Yang, Yung-Chang Chen*. Urinary Biomarkers Improve the Diagnosis of Intrinsic Acute Kidney Injury in Coronary Care units. Medicine. 2015 Oct;94(40):e1703.
  21. Chih-Hsiang Chang, Pei-Chun Fan, Chan-Yu Lin, Chia-Hung Yang, Yi-Ting Chen, Su-Wei Chang, Huang-Yu Yang, Chang-Chyi Jenq, Cheng-Chieh Hung, Chih-Wei Yang, and Yung-Chang Chen. Elevation of Interleukin-18 Correlates with Cardiovascular, Cerebrovascular, and Peripheral Vascular Events: A Cohort Study of Hemodialysis Patients. Medicine. 2015 Oct;94(42):e1836.
  22. Cheng-Hung Lee , Kuo-Sheng Liu , Shang-Hung Chang , Wei-Jan Chen , Kuo-Chun Hung , Shih-Jung Liu* , Jong-Hwei S. Pang , Jyuhn-Huarng Juang , Chou Chung-Chuan , Po-Cheng Chang , Yi-Ting Chen , Fu-Shing Wang. Promoting Diabetic Wound Therapy Using Biodegradable rhPDGF-loaded Nanofibrous Membranes: CONSORT-Compliant Article. Medicine. 2015 Nov;94(47):e1873.
  23. Chia-Hung Yang, Chih-Hsiang Chang, Tien-Hsing Chen, Pei-Chun Fan, Su-Wei Chang, Chun-Chi Chen, Pao-Hsien Chu, Yi-Ting Chen, Huang-Yu Yang, Chih-Wei Yang, Yung-Chang Chen. Combination of Urinary Biomarkers Improve Early Detection of Acute Kidney Injury in Patients with Heart Failure. Circulation Journal, Accepted, 2016.

專書

  1. Yi-Ting Chen, Carol E. Parker, Hsiao-Wei Chen, Chien-Lun Chen, Dominik Domanski, Derek S. Smith, Chih-Ching Wu, Ting Chung, Kung-Hao Liang, Min-Chi Chen, Yu-Sun Chang, Christoph H. Borchers, and Jau-Song Yu. " Comprehensive Biomarker Discovery and Validation for Clinical Application- A Pipeline that Integrates the Discovery and Verification Studies of Urinary Protein Biomarkers Reveals Candidate Markers for Bladder Cancer". Royal Society of Chemistry (RSC). 2013,0, 271-314. ISBN: 978-1-84973-422-6.

專利

發表國內外(台灣,中美日歐盟等多國)專利共計15篇

 

專利名稱

國別

專利號碼

專利期間

膀胱癌之尿液生物標記

中華民國
(ROC)

I390204

20130321~20300210

尿液生物標記作為預測膀胱癌及腎臟癌的用途

中華民國
(ROC)

I522367

20160221~20330411

生物感測器及檢測樣品中待測物濃度的方法

中華民國
(ROC)

I539152

20160621~20350513